Explore our sensory tissue high throughput assay analyses

Human and mouse DRG transcriptome profiles contrasted with other tissues How similar are human and mouse DRG transcriptome profiles ? Which DRG-specific genes are expressed in neuronal subpopulations, and which are expressed in glial subpopulations ? What are the underlying transcription factor based regulatory program that drive these changes ? Are there axonally transported mRNAs in the DRG sensory neurons ? Ray, Pradipta; Torck, Andrew; Quigley, Lilyana; et al; Comparative transcriptome profiling of the human and mouse dorsal root ganglia: an RNA-seq-based resource for pain and sensory neuroscience research. PAIN, 159(7):1325 - 1345, Wolters Kluwer, July 2018. Journal DOI link.	Translational profiling of rodent nociceptors under a Chemotherapy Induced Peripheral Neuropathy model What are the transcriptional and translational changes that are undergone in mouse DRG in response to Paclitaxel treatment ? What are the functional consequences of translational changes ? What are putative regulatory programs driving such change ? Megat, Salim; Ray, Pradipta; Moy, Jamie; et al; Nociceptor translational profiling reveals the Ragulator-Rag GTPase complex as a critical generator of neuropathic pain. Journal of Neuroscience, 2019, 39 (3) 393-411. Journal DOI link.	Transcriptome profiling of human DRG samples with and without associated pain What are the transcriptomic correlates of neuropathic pain ? How do transcriptional changes affect individual patients ? What pathways are turned on / off ? Are there sex specific differences ? North, Robert; Li, Yan; Ray, Pradipta; et al; Electrophysiological and transcriptomic correlates of neuropathic pain in human primary sensory neurons, Brain, 2019, Brain 142 (5), 1215-1226. Journal DOI link .
Sex differences in the human tibial nerve transcriptome How similar are human male and female mRNA profiles in the tibial nerve ? Are the sex-differentially expressed genes involved in nociception, pain, inflammation, immune function or a combinaton of these ? Does the sex dimorphic abundance of transcripts have its basis in glial cells, immune cells, peri- and epi-neurial cells, or neuronal axonally transported transcripts ? Ray,P. et al (2018). Sex-differential gene expression in the human tibial nerve involves key genes implicated in pain, inflammation, and neuro-immunity, Frontiers in Neuroscience, 2019, Journal DOI link.	Transcriptome differences between cultured DRGs in human and mouse How similar are human DRG tissue cultures compared to the in vivo tissue in terms of its RNA profile ? Are there sex differences in the DRG cultures ? Are there species differences in the DRG cultures ? Can we identify genes primarily expressed in post-mitotic cells (and cells dependent on post-mitotic cells for survival) in the mammalian DRG based on the cultured DRG profiles ? Transcriptomic analysis of native versus cultured human and mouse dorsal root ganglia focused on pharmacological targets, Andi Wangzhou, Lisa A McIlvried, Candler Paige, Paulino Barragan-Iglesias, Carolyn A Guzman, Gregory Dussor, Pradipta R Ray, Robert W Gereau, Theodore J Price, 2019, bioRxiv, 766865, biorXiv link